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Human cutaneous γδ T cells, isolated from skin biopsies of HDs and adoptively transferred into recipient mice, infiltrated the human cSCC xenograft from the circulation, homed into the tumor tissue, and were maintained within the tumor. γδ T cells can proliferate following TCR activation and stimulation with IL-1α and IL-18, which are also produced by the tumor tissue. cSCC-infiltrating γδ T cells displayed an activated and cytotoxic phenotype, and infiltration can lead to reduced tumor growth. Figure created in BioRender. I. Gratz (2026) https://BioRender.com/m9vgwti .

Journal: Science Advances

Article Title: Harnessing skin-resident γδ T cells for immunotherapy in cutaneous squamous cell carcinoma

doi: 10.1126/sciadv.aec7215

Figure Lengend Snippet: Human cutaneous γδ T cells, isolated from skin biopsies of HDs and adoptively transferred into recipient mice, infiltrated the human cSCC xenograft from the circulation, homed into the tumor tissue, and were maintained within the tumor. γδ T cells can proliferate following TCR activation and stimulation with IL-1α and IL-18, which are also produced by the tumor tissue. cSCC-infiltrating γδ T cells displayed an activated and cytotoxic phenotype, and infiltration can lead to reduced tumor growth. Figure created in BioRender. I. Gratz (2026) https://BioRender.com/m9vgwti .

Article Snippet: Subsequently, in vitro expanded γδ T cells were isolated by magnetic cell sorting via positive selection, using biotin labeled anti-human TCRγδ antibody (Miltenyi Biotec, catalog no. 130-113-502, AB_2733575) to obtain highly purified γδ T cells (>90%).

Techniques: Isolation, Activation Assay, Produced